Sample preparation/dilution	Non selective/selective enrichment	Isolation, identification and/or enumeration	Confirmation
Bile tolerant gram negative bacteria	Sodium chloride peptone broth (buffered)	EE Broth-MOSSEL (acc. harm. EP/USP/JP) Tryptic Soy Broth	VRBD agar VRBD agar VRBD-agar
Candida albicans	Sodium chloride peptone broth (buffered)	SABOURAUD-2% dextrose broth	SABOURAUD 4% dextrose agar
Clostridia	Sodium chloride peptone broth (buffered)	Sodium chloride peptone broth (buffered)	Columbia agar (base) Reinforced clostridial medium (RCM)
Escherichia coli	Sodium chloride peptone broth (buffered)	MacCONKEY broth Tryptic Soy Broth	MacCONKEY agar
Pseudomonas aeruginosa	Sodium chloride peptone broth (buffered)	Tryptic Soy Broth	Cetrimide Agar (Pseudomonas selective agar) Cetrimide agar
Salmonella	Sodium chloride peptone broth (buffered)	Rappaport-Vassiliadis Salmonella Enrichment Broth (acc. harm. EP/USP/JP) Tryptic Soy Broth	XLD Agar (acc. harm. EP/USP/JP)
Staphylococcus aureus	Sodium chloride peptone broth (buffered)	Tryptic Soy Broth	Mannitol salt phenol-red agar
Total aerobic microbial count	Sodium chloride peptone broth (buffered)	Tryptic Soy Broth	CASO agar CASO agar Tryptic Soy agar with Neutralizers Tryptic soy agar
Total yeast and mould count	Sodium chloride peptone broth (buffered)	SABOURAUD-2% dextrose broth	SABOURAUD 4% dextrose agar with neutralizers SABOURAUD 4% dextrose agar