Purospher® High-purity Columns
The all-round solution for HPLC separations
The key component for modern RP-HPLC sorbents is a high purity silica as starting material. Purospher® HPLC columns are based on high purity, metal-free silica for outstanding separations with excellent peak symmetry.
The base silica for Purospher® high purity HPLC columns is made from tetraalkoxysilane in a sol gel-process. Due to the absence of metals in the silica matrix and combined with an optimized surface coating and shielding process, Purospher® columns provide tailing-free separations of acidic, basic, and chelating compounds. This is of particular advantage for any kind of method development in Research and Development (R&D) and Quality Control (QC) laboratories.
| Purospher® HPLC columns provide: |
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The Purospher® product family. To choose the appropriate column for your HPLC separation, please refer to the specifications.
- Purospher® RP-18 is polar endcapped and suitable for separations of strong basic or chelating compounds
(no acidic compounds) and separations of hydrophilic compounds with a high percentage of water in the mobile phase. - Purospher® RP-18 endcapped is suitable for separations of complex samples with simple eluents.
- Purospher® RP-18 HC is not endcapped with very good suitability for separation of polar, not basic
compounds e.g. explosives. - Purospher® STAR RP-18 endcapped allows the tailing-free separation of neutral, acidic, basic or chelating compounds. The excellent stability up to pH 10.5 enables the separation of strong basic compounds with alkaline eluents. Purospher® STAR RP-18 endcapped is available in different particle sizes of 2µm, 3µm and 5µm and as special UHPLC columns.
- Purospher® STAR RP-8 endcapped for separation of polar compounds or separation of position isomeric compounds.
- Purospher® STAR NH2 and Si for Normal phase Chromatography (Si) and separation of carbohydrates (NH2)
The different selectivities of Purospher® materials are clearly described by the Engelhardt test as well as the Tanaka test.
| Specifications | ||||
|---|---|---|---|---|
| Purospher® STAR RP-18 endcapped |
Purospher® STAR RP-8 endcapped |
Purospher® STAR Si | Purospher® STAR NH2 | |
|
Particle size µm |
2, 3 or 5 | 3 or 5 | 5 | 5 |
| Pore size Å | 120 | 120 | 120 | 120 |
| Pore volume mL/g | 1.1 | 1.1 | 1.1 | 1.1 |
| Specific surface area m²/g |
330 | 330 | 330 | 330 |
| % C load | 17 | 11.2 | - | 3.5 |
| Endcapping | hydrophobic | hydrophobic | no endcapping | hydrophobic |
| pH stability | 1.5 - 10.5 | 1.5 - 10.5 | - | 2 - 7.5 |
| USP classification | L1 | L7 | L3 | L8 |
| Analytes | neutral, acidic, basic or chelating compounds, pH stability |
polar compounds, position isomers |
normal phase chromatography |
carbohydrates |
| Purospher® RP-18 endcapped |
Purospher® RP-18 |
Purospher® RP-18 HC |
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| Particle size µm | 5 | 5 | 5 | |
| Pore size Å | 120 | 90 | 90 | |
| Pore volume mL/g | 1.0 | 0.95 | 1.0 | |
| Specific surface area m²/g |
350 | 500 | > 470 | |
| % C load | 18.0 | 18.5 | 18.0 | |
| Endcapping | no endcapping | amino endcapping | no endcapping | |
| pH stability | 2 - 8 | 2 - 7.5 | 2 - 7.5 | |
| USP classification | L1 | L1 | L1 | |
| Analytes | acids, basic or chelating compounds |
strong bases or chelating compounds |
explosives | |
