Information
X


Merck Chemicals

more less

Purospher® - High-purity HPLC columns

Purospher® STAR Si and Purospher® STAR NH2

Purospher® STAR RP-8 endcapped

Purospher® STAR HPLC columns based on highest purity silica are also available for normal phase separations.

Purospher® STAR Si (Silica) offers highest separation efficiency for normal-phase chromatography of low molecular weight compounds soluble in organic solvents.

Purospher® STAR NH2 (Amino) is primarily designed for carbohydrate analysis with a typical mobile phase consisting of acetonitrile and water. Additionally, Purospher® STAR NH2 can also be used in the normal-phase retention mode.

 

Key performance benefits:
  • Very high separation efficiency as measured by the plate count
  • Absence of metal impurities, thus giving consistently symmetrical peaks
  • Extended column lifetime
Specification of Purospher® STAR Si and Purospher® STAR NH2
 Purospher® STAR SiPurospher® STAR NH2
Sorbent characteristics High purity silica gel With NH2 (amino) modification
Metal content Na, Ca, Mg, Al: 1 ppm; Fe: 3 ppm Na, Ca, Mg, Al: 1 ppm; Fe: 3 ppm
Particle shape spherical spherical
Particle size 5 μm 5 μm
Pore size 120 A (12 nm) 120 A (12 nm)
Pore volume 1.1 mL/g 1.1 mL/g
Spec. surface area 330 m²/g 330 m²/g
Carbon load   3.5 %
Coverage of the surface 3 µmol/m² 3 µmol/m²
pH range pH 2-7.5 pH 2-7.5
Efficiency > 50.000 N/m > 50.000 N/m
Shipping eluent n-Heptane n-Heptane
Separation of Carbohydrates on Purospher® STAR NH2
Column LiChroCART® 250-4 Purospher® STAR NH2, 5 µm Separation of Carbohydrates on Purospher® STAR NH2
Eluent Acetonitrile/Water (75:25)
Flow rate   1.0 mL/min
Detection RI
Temperature 30°C
Inj. volume 10 µL
Sample 1. Xylose
2. Fructose
3. Glucose
4. Saccharose
5. Maltose
6. Lactose
Separation of Anisoles on Purospher® STAR Silica
Column LiChroCART® 125-4 Purospher® STAR Si, 5 µm Separation of Anisoles on Purospher® STAR Silica
Eluent n-Heptane/Dioxane (95/5, v/v)
Flow rate   2.0 mL/min
Detection UV 254 nm fast response
Temperature RT
Inj. volume 5 µL
Sample

1. Anisole
2. 3-Nitroanisole
3. 4-Nitroanisole
4. 2-Nitroanisole

 

© Merck KGaA, Darmstadt, Germany, 2012


x

Welcome to Merck Millipore

 

Europe, Middle East and Africa Asia / Pacific North America, Central America and the Caribbean South America

Please choose your country:

North America, Central America and the Caribbean

South America

Europe, Middle East and Africa

Asia / Pacific

All countries from A to Z

If you can't find your country please click here.

 

x

Merck Chemicals Cart ()

You are entering the Millipore online shop. Both carts are maintained for you while you shop between sites. We recommend to complete your Merck order before visiting Millipore.com.

Go to Millipore Proceed to checkout


What kind of products are you looking for?

Millipore

Bio manufacturing and Life science.

Merck Chemicals

High-quality industrial and laboratory chemicals.


Important message


It is currently not possible to order Bioscience products together with other Merck products. We recommend completing your current order first.

Do you wish to proceed without completing your current order? If so, this will empty your shopping cart.